BEGIN:VCALENDAR
VERSION:2.0
PRODID:-//UM//UM*Events//EN
CALSCALE:GREGORIAN
BEGIN:VTIMEZONE
TZID:America/Detroit
TZURL:http://tzurl.org/zoneinfo/America/Detroit
X-LIC-LOCATION:America/Detroit
BEGIN:DAYLIGHT
TZOFFSETFROM:-0500
TZOFFSETTO:-0400
TZNAME:EDT
DTSTART:20070311T020000
RRULE:FREQ=YEARLY;BYMONTH=3;BYDAY=2SU
END:DAYLIGHT
BEGIN:STANDARD
TZOFFSETFROM:-0400
TZOFFSETTO:-0500
TZNAME:EST
DTSTART:20071104T020000
RRULE:FREQ=YEARLY;BYMONTH=11;BYDAY=1SU
END:STANDARD
END:VTIMEZONE
BEGIN:VEVENT
DTSTAMP:20250326T093027
DTSTART;TZID=America/Detroit:20250408T160000
SUMMARY:Workshop / Seminar:The Radical Lives of Anaerobes and How to Fight Them
DESCRIPTION:Pathogenic bacteria that infect the gastrointestinal and respiratory tract form biofilms that are largely anaerobic\, modulating their metabolic lifestyle and virulence. Many of these microbes utilize acetate fermentation via the enzyme pyruvate formate lyase (PFL)\, which catalyzes the production of acetyl-CoA and formate from pyruvate via a radical mechanism\, enabling additional ATP production and the de novo synthesis of proteins and nucleic acids. We have investigated the mechanism of PFL via mechanism-based inhibitors to reveal the essential role of two cysteine thiyl radicals in catalysis and estimate their reactivity for the first time. Additionally\, we have demonstrated the role of nitric oxide (NO) as an irreversible inhibitor of they glycyl radical enzyme PFL as well as glycyl radical enzymes as a class and their radical S-adenosylmethionine-dependent activases via the production of dinitrosyl iron complexes. These results set the stage for the development of mechanism-based inhibitors of PFL and other glycyl radical enzymes and provide a biochemically informed approach to narrow-spectrum site-specific antibiotic development for the treatment of gastrointestinal and pulmonary disease.
UID:125093-21854406@events.umich.edu
URL:https://events.umich.edu/event/125093
CLASS:PUBLIC
STATUS:CONFIRMED
CATEGORIES:Chemical Biology,Chemistry
LOCATION:Chemistry Dow Lab - 1640
CONTACT:
END:VEVENT
END:VCALENDAR