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DTSTART:20070311T020000
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DTSTAMP:20260909T100435
DTSTART;TZID=America/Detroit:20261008T160000
DTEND;TZID=America/Detroit:20261008T171500
SUMMARY:Workshop / Seminar:From Spontaneous to Stimulated Emission: Quantum Optical Probes for Supramolecular Organization
DESCRIPTION:Quantum statistical properties of fluorescence emitted spontaneously in random directions underpin a variety of experimental methods for determining molecular interactions in both test tubes and biological cells. I will begin this talk with a brief overview of a method developed in my lab\, dubbed Fluorescence Intensity Fluctuation (FIF) Spectrometry\, which probes spatial fluctuations in fluorescence intensities from fluorescently labeled proteins to determine the number of subunits comprising a complex. I will then present Förster Resonance Energy Transfer (FRET) Spectrometry and its application to determination of the relative distances between and orientation of fluorescent molecules within a complex via energy transfer between their transition dipoles. In the third part of the talk\, I will introduce a mathematical framework for quantization of electromagnetic fields emitted by single dipole radiators\, which predicts both the directionality of photon emission probability and light-stimulated electromagnetic wave collapse. I will conclude by presenting preliminary experimental results testing these effects. These quantum phenomena could lead to new methods for directly determining protein orientation within complexes\, complementing the spontaneous emission-based methods.
UID:151811-21912212@events.umich.edu
URL:https://events.umich.edu/event/151811
CLASS:PUBLIC
STATUS:CONFIRMED
CATEGORIES:Chemistry,Physical Chemistry
LOCATION:Chemistry Dow Lab - CHEM 1640
CONTACT:
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