{
    "146965-21899887":
    {
        "datetime_modified":"20260325T140233",
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        "date_start":"2026-05-28",
        "date_end":"2026-05-28",
        "time_start":"16:00:00",
        "time_end":"17:15:00",
        "time_zone":"America\/Detroit",
        "event_title":"Co-Fractionation \u2018Multi-omics\u2019 Mass Spectrometry for Lipid-Protein Interactome Analysis",
        "occurrence_title":"",
        "combined_title":"Co-Fractionation \u2018Multi-omics\u2019 Mass Spectrometry for Lipid-Protein Interactome Analysis: Gavin Reid (University of Melbourne)",
        "event_subtitle":"Gavin Reid (University of Melbourne)",
        "event_type":"Workshop \/ Seminar",
        "event_type_id":"21",
        "description":"Lipids play essential roles in regulating various biological processes through functional interactions with integral or peripherally associated proteins and protein complexes. While mass spectrometry (MS)-based strategies have enabled global lipidome and proteome identification and quantitative profiling, high-throughput strategies to investigate the lipid-protein \u2018interactome\u2019 at the systems level are currently lacking. Here, I will first describe the development of advanced \u2018shotgun\u2019 lipidomics data acquisition workflows, including the use of ion-mobility for selective enrichment of low abundance lipid classes, and UV-photodissociation MS\/MS to enable complete lipid structural characterisation [e.g., Anal. Chem. 2024, 96, 12296-12307.; Anal. Bioanal. Chem. 2020, 412, 2339\u20132351.], and their application to characterise a novel cause of autosomal regressive multisystem mitochondrial disease resulting from deleterious variants in cardiolipin synthase 1 (CRLS1) [Human Mol. Genetics. 2022, 31, 3597-3612.]. Next, using an integrated \u2018multi-omics\u2019 workflow for comprehensive lipidome and proteome profiling, I will report that the knockout of non-mitochondrial genes in peroxisome, endoplasmic reticulum (ER) and Golgi organelles also results in mitochondrial dysfunction, caused by unexpected alterations in ether-glycerophospholipid metabolic pathways [Nature. Cell Biol. 2024, 26, 57-71]. Finally, to investigate the lipid\u2013protein interaction relationships in these systems, a novel Co-Fractionation Multi-Omics Mass Spectrometry (CF-MOMS) strategy will be described to characterise global alterations in the lipid-protein \u2018interactome interactomes in WT and CRLS1KO cell lines, and to highlight the functional involvement of cardiolipin-protein interactions in maintaining the assembly\/stability and activity of mitochondrial membrane proteins.",
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        "location_name":"Chemistry Dow Lab",
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        "tags":["Analytical Chemistry","Chemistry"],
        "website":"https:\/\/findanexpert.unimelb.edu.au\/profile\/28892-gavin-reid",
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