[ { "id":"140436-21887150", "datetime_modified":"20251008T121103", "datetime_start":"20251024T160000", "datetime_end":"20251024T170000", "has_end_time":1, "date_start":"2025-10-24", "date_end":"2025-10-24", "time_start":"16:00:00", "time_end":"17:00:00", "time_zone":"America\/Detroit", "event_title":"CCMB\/DCMB Special Seminar featuring Hagen Tilgner, PhD (Prof. at Weil Cornell Medical College)", "occurrence_title":"", "combined_title":"CCMB\/DCMB Special Seminar featuring Hagen Tilgner, PhD (Prof. at Weil Cornell Medical College): \"A single-cell view of splicing (dys)regulation across in development and the diseases of the brain.\"", "event_subtitle":"\"A single-cell view of splicing (dys)regulation across in development and the diseases of the brain.\"", "event_type":"Workshop \/ Seminar", "event_type_id":"21", "description":"Abstract\r\nComplex tissue includes diverse cell types employing distinct RNA isoforms. To untangle full-length cell-type specific brain isoforms, we developed single-cell long-read technology for many thousands of cells (from previous approaches for 10-100 cells) in fresh tissues (ScISOr-Seq; Gupta..Tilgner, 20181) and in frozen tissues (SnISOr-Seq; Hardwick..Tilgner, 20222). These approaches revealed the rules of combination of TSSs, alternative exons and poly(A) sites and their cell-type specificity. Autism-associated exons (as previously described) but also FTD-associated exons are highly variably-used across cell types2. For spatial resolution, we developed spatially-barcoded isoform sequencing with 60um (Joglekar..Tilgner, 20213), 10um (Foord..Tilgner, 20254) and 220nm (Michielsen..Tilgner, biorxiv5) spots, showing that often isoform switches correlate with precise boundaries of brain structures (e.g., choroid plexus to hippocampus). However, genes including Snap25, use a gradient of exon inclusion through the brain3. Choroid plexus epithelial cells show a dramatically distinct isoform profile, which originates most strongly from TSS usage3. During human puberty, layer4-excitatory splicing is more regulated than in other cortical layers \u2013 probably influenced by retroviral sequences4. More generally, we can now detect isoform-expression variability that does not correspond to known brain structures5.\r\n\r\n\r\nFor the NIH Brain Initiative, we have mapped single-cell isoforms across development, brain regions and species. Neurotransmitter release and reuptake as well as synapse turnover genes harbor variability in the same cell type across anatomical regions but the same cell type traced across development shows more isoform variability than across adult anatomical regions. Moreover, most cell-type-specific exons in adult mouse hippocampus behave similarly in human hippocampi. However, human brains have evolved additional cell-type specificity in splicing (Joglekar..Tilgner, 20246). Additionally, the concurrent measurement of chromatin and splicing patterns in post-mortem human brain shows broadly-speaking convergent dysregulation of both modalities in similar cell types in Alzheimer\u2019s disease but more divergence between both modalities in evolution (Hu..Tilgner, 20257). Finally, we have advanced our understanding of error sources of PacBio and ONT (Mikheenko..Tilgner, 20228) and implemented highly accurate long-read software (Prjibelski..Tilgner, 20239).", "occurrence_notes":null, "guid":"140436-21887150@events.umich.edu", "permalink":"http:\/\/events.umich.edu\/event\/140436", "building_id":"1000209", "building_name":"Taubman Library", "building_official_id":1000209, "campus_maps_id":"149", "campus_maps_link_path":"alfred-a-taubman-health-sciences-library", "campus_maps_link":"https:\/\/maps.studentlife.umich.edu\/building\/alfred-a-taubman-health-sciences-library", "room":"2901", "location_name":"Taubman Library", "has_livestream":0, "cost":"", "tags":["Bioinformatics","Life Science","Biosciences","Biology","Basic Science"], "website":"https:\/\/umich.zoom.us\/j\/93929606089?pwd=SHh6R1FOQm8xMThRemdxTEFMWWpVdz09", "links":[ { "type":"livestream", "url":"https:\/\/umich.zoom.us\/j\/93929606089?pwd=SHh6R1FOQm8xMThRemdxTEFMWWpVdz09", "title":"ZOOM Livestream" }, { "type":"other", "url":"https:\/\/www.tilgnerlab.com\/", "title":"Tilgner Lab" }, { "type":"other", "url":"https:\/\/medschool.umich.edu\/departments\/computational-medicine-bioinformatics\/ccmb-seminar-hagen-tilgner-phd", "title":"Event Webpage" } ], "sponsors":[ { "group_name":"Department of Computational Medicine and Bioinformatics DCMB", "group_id":"5384", "website":"" }, { "group_name":"DCMB Seminar Series", "group_id":"3754", "website":"" } ], "image_url":"https:\/\/events.umich.edu\/media\/attachments\/2025\/10\/event_140436_original-1.jpg", "image_description":"Hagen Tilgner, PhD", "styled_images":{ "event_thumb":"https:\/\/events.umich.edu\/media\/cache\/event_thumb\/media\/attachments\/2025\/10\/event_140436_original-1.jpg", "event_large":"https:\/\/events.umich.edu\/media\/cache\/event_large\/media\/attachments\/2025\/10\/event_140436_original-1.jpg", "event_large_2x":"https:\/\/events.umich.edu\/media\/cache\/resolve\/event_large_2x\/media\/attachments\/2025\/10\/event_140436_original-1.jpg", "event_large_lightbox":"https:\/\/events.umich.edu\/media\/cache\/event_large_lightbox\/media\/attachments\/2025\/10\/event_140436_original-1.jpg", "group_thumb":"https:\/\/events.umich.edu\/media\/cache\/resolve\/group_thumb\/media\/attachments\/2025\/10\/event_140436_original-1.jpg", "group_thumb_square":"https:\/\/events.umich.edu\/media\/cache\/resolve\/group_thumb_square\/media\/attachments\/2025\/10\/event_140436_original-1.jpg", "group_large":"https:\/\/events.umich.edu\/media\/cache\/resolve\/group_large\/media\/attachments\/2025\/10\/event_140436_original-1.jpg", "group_large_lightbox":"https:\/\/events.umich.edu\/media\/cache\/group_large_lightbox\/media\/attachments\/2025\/10\/event_140436_original-1.jpg", "event_large_crop":"https:\/\/events.umich.edu\/media\/cache\/resolve\/event_large_crop\/media\/attachments\/2025\/10\/event_140436_original-1.jpg", "event_list":"https:\/\/events.umich.edu\/media\/cache\/event_list\/media\/attachments\/2025\/10\/event_140436_original-1.jpg", "event_list_2x":"https:\/\/events.umich.edu\/media\/cache\/event_list_2x\/media\/attachments\/2025\/10\/event_140436_original-1.jpg", "event_grid":"https:\/\/events.umich.edu\/media\/cache\/event_grid\/media\/attachments\/2025\/10\/event_140436_original-1.jpg", "event_grid_2x":"https:\/\/events.umich.edu\/media\/cache\/resolve\/event_grid_2x\/media\/attachments\/2025\/10\/event_140436_original-1.jpg", "event_feature_large":"https:\/\/events.umich.edu\/media\/cache\/resolve\/event_feature_large\/media\/attachments\/2025\/10\/event_140436_original-1.jpg", "event_feature_thumb":"https:\/\/events.umich.edu\/media\/cache\/resolve\/event_feature_thumb\/media\/attachments\/2025\/10\/event_140436_original-1.jpg" }, "qr_code_url":"http:\/\/events.umich.edu\/event\/140436\/qrcode.png", "occurrence_count":1, "first_occurrence":21887150 } , { "id":"146600-21899337", "datetime_modified":"20260314T102550", "datetime_start":"20260412T120000", "datetime_end":"20260412T150000", "has_end_time":1, "date_start":"2026-04-12", "date_end":"2026-04-12", "time_start":"12:00:00", "time_end":"15:00:00", "time_zone":"America\/Detroit", "event_title":"AAPI Community Health Screening", "occurrence_title":"", "combined_title":"AAPI Community Health Screening", "event_subtitle":"", "event_type":"Well-being", "event_type_id":"12", "description":"United Asian American Medical Student Association (UAAMSA) is hosting our annual Community Health Screening to help combat barriers that the local Asian population may encounter in managing their health. We will be providing free blood pressure measurements, A1c blood sugar, and eye exams as well as education on interpreting screening results. Mandarin-speaking interpreters and materials will also be provided.", "occurrence_notes":null, "guid":"146600-21899337@events.umich.edu", "permalink":"http:\/\/events.umich.edu\/event\/146600", "building_id":"1000209", "building_name":"Taubman Library", "building_official_id":1000209, "campus_maps_id":"149", "campus_maps_link_path":"alfred-a-taubman-health-sciences-library", "campus_maps_link":"https:\/\/maps.studentlife.umich.edu\/building\/alfred-a-taubman-health-sciences-library", "room":"", "location_name":"Taubman Library", "has_livestream":0, "cost":"", "tags":["Asia","Life Science","Medicine"], "website":"", "links":[ ], "sponsors":[ { "group_name":"United Asian American Medical Student Association", "group_id":"5495", "website":"" } ], "image_url":"https:\/\/events.umich.edu\/media\/attachments\/2026\/03\/event_146600_original-1.png", "image_description":"UAAMSA members at our last health screening", "styled_images":{ "event_thumb":"https:\/\/events.umich.edu\/media\/cache\/event_thumb\/media\/attachments\/2026\/03\/event_146600_original-1.png", "event_large":"https:\/\/events.umich.edu\/media\/cache\/event_large\/media\/attachments\/2026\/03\/event_146600_original-1.png", "event_large_2x":"https:\/\/events.umich.edu\/media\/cache\/event_large_2x\/media\/attachments\/2026\/03\/event_146600_original-1.png", "event_large_lightbox":"https:\/\/events.umich.edu\/media\/cache\/event_large_lightbox\/media\/attachments\/2026\/03\/event_146600_original-1.png", "group_thumb":"https:\/\/events.umich.edu\/media\/cache\/group_thumb\/media\/attachments\/2026\/03\/event_146600_original-1.png", "group_thumb_square":"https:\/\/events.umich.edu\/media\/cache\/group_thumb_square\/media\/attachments\/2026\/03\/event_146600_original-1.png", "group_large":"https:\/\/events.umich.edu\/media\/cache\/group_large\/media\/attachments\/2026\/03\/event_146600_original-1.png", "group_large_lightbox":"https:\/\/events.umich.edu\/media\/cache\/group_large_lightbox\/media\/attachments\/2026\/03\/event_146600_original-1.png", "event_large_crop":"https:\/\/events.umich.edu\/media\/cache\/event_large_crop\/media\/attachments\/2026\/03\/event_146600_original-1.png", "event_list":"https:\/\/events.umich.edu\/media\/cache\/event_list\/media\/attachments\/2026\/03\/event_146600_original-1.png", "event_list_2x":"https:\/\/events.umich.edu\/media\/cache\/event_list_2x\/media\/attachments\/2026\/03\/event_146600_original-1.png", "event_grid":"https:\/\/events.umich.edu\/media\/cache\/event_grid\/media\/attachments\/2026\/03\/event_146600_original-1.png", "event_grid_2x":"https:\/\/events.umich.edu\/media\/cache\/event_grid_2x\/media\/attachments\/2026\/03\/event_146600_original-1.png", "event_feature_large":"https:\/\/events.umich.edu\/media\/cache\/event_feature_large\/media\/attachments\/2026\/03\/event_146600_original-1.png", "event_feature_thumb":"https:\/\/events.umich.edu\/media\/cache\/event_feature_thumb\/media\/attachments\/2026\/03\/event_146600_original-1.png" }, "qr_code_url":"http:\/\/events.umich.edu\/event\/146600\/qrcode.png", "occurrence_count":1, "first_occurrence":21899337 } ]