{ "120687-21845136": { "datetime_modified":"20240404T095257", "datetime_start":"20240412T160000", "datetime_end":"20240412T171500", "has_end_time":1, "date_start":"2024-04-12", "date_end":"2024-04-12", "time_start":"16:00:00", "time_end":"17:15:00", "time_zone":"America\/Detroit", "event_title":"New tools for flow-based peptide and protein synthesis", "occurrence_title":"", "combined_title":"New tools for flow-based peptide and protein synthesis: Nina Hartrampf (University of Zurich)", "event_subtitle":"Nina Hartrampf (University of Zurich)", "event_type":"Workshop \/ Seminar", "event_type_id":"21", "description":"The protein MYC is an intrinsically disordered transcription factor that is upregulated in>50% of cancers and engages in numerous protein-protein interactions. [1] These interactions\r\nare often regulated through posttranslational modifications (PTMs) within MYC\u2019s N-terminus\r\n(transactivation domain), most commonly (poly)phosphorylation. Studying these interactions\r\non a molecular level requires proteins with unique and defined PTM patterns, which are\r\nchallenging to obtain by recombinant methods. [2] Flow-based solid-phase peptide synthesis\r\n(SPPS) could therefore be used to obtain long, uniquely modified MYC peptides to study\r\nPTM-dependent binding interactions on a molecular level. [3,4]\r\nA challenge that arose during the chemical synthesis of MYC\u2019s transactivation domain\u2014that\r\nis often encountered in SPPS\u2014was the aggregation of growing peptide chains (\"difficult\r\nsequences\u201d), which can lead to incomplete couplings. Previous research into this sequence-\r\ndependent phenomenon was limited by the lack of high-throughput analytical methods,\r\nthus impeding systematic analysis. While flow-based SPPS allows for aggregation detection,\r\nit has so far not led to the development of tools for its suppression.\r\nTo enable the synthesis of MYC\u2019s transactivation domain, we developed a \u201cSynthesis Tag\u201d\r\n(SynTag) consisting of six arginines connected via a cleavable MeDbz linker. [5] SynTag\r\neffectively improves batch- and flow-SPPS of \u201cdifficult sequences\u201d, enhances the solubility of\r\nthe cleaved peptides and provides direct access to native sequences by hydrolysis, or\r\npeptide thioesters for Native Chemical Ligation (NCL). We demonstrate its utility in the first\r\nchemical synthesis of the MYC transactivation domain with a single NCL. We envisage\r\nSynTag to become a broadly applicable tool that enables the synthesis and study of\r\npreviously unattainable peptides and proteins.\r\n\r\n[1] C. Lourenco, D. Resetca, C. Redel, P. Lin, A. S. MacDonald, R. Ciaccio, T. M. G. Kenney, Y. Wei,\r\nD. W. Andrews, M. Sunnerhagen, C. H. Arrowsmith, B. Raught, L. Z. Penn, Nat. Rev. Cancer\r\n2021, 21, 579\u2013591\r\n[2] A. C. Conibear, Nat. Rev. Chem. 2020, 4, 674\u2013695; T. Bilbrough, E. Piemontese, O. Seitz, Chem.\r\nSoc. Rev. 2022, 51, 5691\u20135730\r\n[3] N. Hartrampf, A. Saebi, M. Poskus, Z. P. Gates, A. J. Callahan, A. E. Cowfer, S. Hanna, S.\r\nAntilla, C. K. Schissel, A. J. Quartararo, X. Ye, A. J. Mijalis, M. D. Simon, A. Loas, S. Liu, C.\r\nJessen, T. E. Nielsen, B. L. Pentelute, Science 2020, 368, 980.\r\n[3] E. T. Williams, K. Schiefelbein, M. Schuster, I. M. M. Ahmed, M. De Vries, R. Beveridge, O.\r\nZerbe, N. Hartrampf, ChemRxiv 2024, DOI: 10.26434\/chemrxiv-2024-mfpkx.\r\n[4] H. B\u00fcrgisser, E. T. Williams, R. Lescure, A. Premanand, A. Jeandin, N. Hartrampf, ChemRxiv\r\n2023, DOI: 10.26434\/chemrxiv-2023-7mz2c.", "occurrence_notes":null, "guid":"120687-21845136@events.umich.edu", "permalink":"http:\/\/events.umich.edu\/event\/120687", "building_id":"1000158", "building_name":"Chemistry Dow Lab", "campus_maps_id":"34", "room":"1706", "location_name":"Chemistry Dow Lab", "has_livestream":0, "cost":"", "tags":["Chemistry"], "website":"", "sponsors":[ { "group_name":"Department of Chemistry", "group_id":"3761", "website":"https:\/\/lsa.umich.edu\/chem" } ], "image_url":"", "styled_images":{ "event_thumb":"", "event_large":"", "event_large_2x":"", "event_large_lightbox":"", "group_thumb":"", "group_thumb_square":"", "group_large":"", "group_large_lightbox":"", "event_large_crop":"", "event_list":"", "event_list_2x":"", "event_grid":"", "event_grid_2x":"", "event_feature_large":"", "event_feature_thumb":"" }, "occurrence_count":1, "first_occurrence":21845136 } , "118322-21840884": { "datetime_modified":"20240202T164100", "datetime_start":"20240412T160000", "datetime_end":"20240412T173000", "has_end_time":1, "date_start":"2024-04-12", "date_end":"2024-04-12", "time_start":"16:00:00", "time_end":"17:30:00", "time_zone":"America\/Detroit", "event_title":"Preprint Algebraic Geometry Seminar: Du Bois complex and extension of forms after Park", "occurrence_title":"", "combined_title":"Preprint Algebraic Geometry Seminar: Du Bois complex and extension of forms after Park: Sridhar Venkatesh (UM)", "event_subtitle":"Sridhar Venkatesh (UM)", "event_type":"Workshop \/ Seminar", "event_type_id":"21", "description":"TBA", "occurrence_notes":null, "guid":"118322-21840884@events.umich.edu", "permalink":"http:\/\/events.umich.edu\/event\/118322", "building_id":"1000166", "building_name":"East Hall", "campus_maps_id":"53", "room":"4096", "location_name":"East Hall", "has_livestream":0, "cost":"", "tags":["Mathematics"], "website":"", "sponsors":[ { "group_name":"Preprint Algebraic Geometry Seminar - Department of Mathematics", "group_id":"4899", "website":"" }, { "group_name":"Department of Mathematics", "group_id":"3791", "website":"" } ], "image_url":"", "styled_images":{ "event_thumb":"", "event_large":"", "event_large_2x":"", "event_large_lightbox":"", "group_thumb":"", "group_thumb_square":"", "group_large":"", "group_large_lightbox":"", "event_large_crop":"", "event_list":"", "event_list_2x":"", "event_grid":"", "event_grid_2x":"", "event_feature_large":"", "event_feature_thumb":"" }, "occurrence_count":1, "first_occurrence":21840884 } , "117510-21839419": { "datetime_modified":"20240119T143313", "datetime_start":"20240412T163000", "datetime_end":"20240412T173000", "has_end_time":1, "date_start":"2024-04-12", "date_end":"2024-04-12", "time_start":"16:30:00", "time_end":"17:30:00", "time_zone":"America\/Detroit", "event_title":"SAPAC BIPOC Peer Led Support Group Winter 2024", "occurrence_title":"", "combined_title":"SAPAC BIPOC Peer Led Support Group Winter 2024", "event_subtitle":"", "event_type":"Well-being", "event_type_id":"12", "description":"BIPOC PLSG (peer led support group), is a drop-in, confidential healing space for survivors of sexual assault, intimate partner violence, stalking, and\/or sexual harassment, who identify as people of color. Facilitated by student staff, BIPOC PLSG is a place for survivors of color at UM to find not only community but healing opportunities, including anxiety-reduction, self-care activities, and mindfulness.\r\n\r\nPOC PLSG offers low-key activities as well as a safe space for sharing experiences with racial\/ethnic identity, violence, and the intersection between both, as people are comfortable sharing. Survivors are welcome whether they experienced harm in college, or earlier in life.\r\n\r\nThis space specifically centers UM student survivors who identify as people of color; if you do not identify as a person of color, we encourage you to consider joining SAPAC\u2019s general Peer Led Support Group: sapac.umich.edu\/PLSG\r\n\r\n \r\n\r\nTo fill out a confidential interest form and receive emails from facilitators: BIPOC PLSG Interest Form: forms.gle\/uW7Nq6FfhoiwvtuL9\r\n\r\nEmail: bipoc-plsg@umich.edu\r\n\r\n \r\n\r\nWinter 2024 Meeting Schedule:\r\n\r\nWhen: \r\n\r\nMondays via Zoom - 5:30-6:30pm (first meeting on Monday Jan 22nd)\r\nFridays in person - 4:30-5:30pm (first meeting on Friday Jan 19th) \r\n\r\nLocation: \r\n\r\nIn person - SAPAC Office, 4100 Michigan Union, Virtual - Zoom", "occurrence_notes":null, "guid":"117510-21839419@events.umich.edu", "permalink":"http:\/\/events.umich.edu\/event\/117510", "building_id":"1000120", "building_name":"Michigan Union", "campus_maps_id":"191", "room":"4100 (SAPAC Shared Space)", "location_name":"Michigan Union", "has_livestream":0, "cost":"", "tags":["free","Health & Wellness","peer education","sapac","Well-being"], "website":"https:\/\/docs.google.com\/forms\/d\/e\/1FAIpQLSfVA46i15-BqBvEseUXHJ2nZJubKBep5_UgpSO2La38iAmcxw\/viewform", "sponsors":[ { "group_name":"Sexual Assault Prevention and Awareness Center (SAPAC)", "group_id":"1688", "website":"http:\/\/sapac.umich.edu" } ], "image_url":"https:\/\/events.umich.edu\/media\/attachments\/2024\/01\/event_117510_original-1.png", "styled_images":{ "event_thumb":"https:\/\/events.umich.edu\/media\/cache\/event_thumb\/media\/attachments\/2024\/01\/event_117510_original-1.png", "event_large":"https:\/\/events.umich.edu\/media\/cache\/event_large\/media\/attachments\/2024\/01\/event_117510_original-1.png", "event_large_2x":"https:\/\/events.umich.edu\/media\/cache\/event_large_2x\/media\/attachments\/2024\/01\/event_117510_original-1.png", "event_large_lightbox":"https:\/\/events.umich.edu\/media\/cache\/event_large_lightbox\/media\/attachments\/2024\/01\/event_117510_original-1.png", "group_thumb":"https:\/\/events.umich.edu\/media\/cache\/group_thumb\/media\/attachments\/2024\/01\/event_117510_original-1.png", "group_thumb_square":"https:\/\/events.umich.edu\/media\/cache\/group_thumb_square\/media\/attachments\/2024\/01\/event_117510_original-1.png", "group_large":"https:\/\/events.umich.edu\/media\/cache\/group_large\/media\/attachments\/2024\/01\/event_117510_original-1.png", "group_large_lightbox":"https:\/\/events.umich.edu\/media\/cache\/group_large_lightbox\/media\/attachments\/2024\/01\/event_117510_original-1.png", "event_large_crop":"https:\/\/events.umich.edu\/media\/cache\/event_large_crop\/media\/attachments\/2024\/01\/event_117510_original-1.png", "event_list":"https:\/\/events.umich.edu\/media\/cache\/event_list\/media\/attachments\/2024\/01\/event_117510_original-1.png", "event_list_2x":"https:\/\/events.umich.edu\/media\/cache\/event_list_2x\/media\/attachments\/2024\/01\/event_117510_original-1.png", "event_grid":"https:\/\/events.umich.edu\/media\/cache\/event_grid\/media\/attachments\/2024\/01\/event_117510_original-1.png", "event_grid_2x":"https:\/\/events.umich.edu\/media\/cache\/event_grid_2x\/media\/attachments\/2024\/01\/event_117510_original-1.png", "event_feature_large":"https:\/\/events.umich.edu\/media\/cache\/event_feature_large\/media\/attachments\/2024\/01\/event_117510_original-1.png", "event_feature_thumb":"https:\/\/events.umich.edu\/media\/cache\/event_feature_thumb\/media\/attachments\/2024\/01\/event_117510_original-1.png" }, "occurrence_count":29, "first_occurrence":21839407 } }