{ "84438-21623984": { "datetime_modified":"20210712T121344", "datetime_start":"20210721T090000", "datetime_end":"20210721T100000", "has_end_time":1, "date_start":"2021-07-21", "date_end":"2021-07-21", "time_start":"09:00:00", "time_end":"10:00:00", "time_zone":"America\/Detroit", "event_title":"BME Ph.D. Defense: Michael Bregenzer", "occurrence_title":"", "combined_title":"BME Ph.D. Defense: Michael Bregenzer: The Role of the Tumor Microenvironment in Cancer Stem Cell Regulation and the Development of Chemoresistance in Ovarian Cancer", "event_subtitle":"The Role of the Tumor Microenvironment in Cancer Stem Cell Regulation and the Development of Chemoresistance in Ovarian Cancer", "event_type":"Presentation", "event_type_id":"16", "description":"High grade serous ovarian cancer (HGSOC) is the deadliest gynecological malignancy. Despite successful first line therapies, most patients relapse and develop more chemoresistant disease. This recurrence and development of chemoresistance is attributed to a rare population of tumor cells, termed cancer stem cells (CSCs), which are more chemoresistant, have the capacity to self-renew, and can repopulate the entire tumor. Research has shown that CSCs are maintained by the non-cancer cells in the tumor microenvironment (TME), such as mesenchymal stem cells (MSCs), endothelial cells (ECs), and immune cells. Furthermore, the role of non-cancer cells in clinical outcomes and chemoresistance has been highlighted by recent evidence showing that classification of HGSOC molecular subtypes, which have variable clinical prognoses, are influenced by the presence of non-cancer cells in the tumor. However, it is currently unclear exactly how CSCs and the nuanced cell composition of the TME work together to promote chemoresistance. Current models used to study these phenomena either suffer from a lack of cellular complexity in the case of many in vitro models or impractical experimental constraints such as long latency periods and poor control over cell composition in patient-derived xenografts. To better understand the role of CSCs and the TME cells in chemoresistance, practical in vitro model systems that more closely represent in vivo processes and microenvironments are needed. We hypothesize that the development of these in vitro model systems will contribute novel insights into TME-mediated CSC regulation and the development of chemoresistance in HGSOC.\r\n\r\nIn aim 1 we examined the emergence of chemoresistance in the context of CSCs by developing a 3D in vitro serial passaging model system that allows for long term culture of patient-derived tumor cells with periodic evaluation of stemness and chemoresistance. Using this model system, we demonstrated increased proliferation, expression of CSC markers, tumorigenicity, and chemoresistance over the course of long-term passaging, reflective of emerging chemoresistance in vivo. Furthermore, this system enabled us to define a malignant gene expression signature that is associated with chemoresistance, tumorigenicity, and stemness and to evaluate patient-specific chemoresistance development following treatment. Finally, we demonstrated the translational value of this model system by showing that Metformin treatment can hinder CSC driven development of chemoresistance in a phase II clinical trial.\r\n\r\nIn aim 2 we developed a heterogeneous tumoroid culture system that enabled culture of patient-derived tumor cells with controlled ratios of MSCs, ECs, and immune cells to study TME-mediated maintenance of CSCs and chemoresistance. Using this model, we found that changes in CD133+\/-ALDH+\/- CSC phenotypes in response to TME cells varied depending on the patient sample. We also observed increased tumorigenicity and chemoresistance in tumoroids compared to spheroids cultured with patient-derived tumor cells alone. Furthermore, we found evidence of epithelial-to-mesenchymal transition (EMT) in tumoroids accompanied by altered CSC phenotypes and a malignant matrisome signature. All of this together supports idea that the non-cancer cells in the TME contribute to the development of advanced, chemoresistant disease and implicates EMT, changes in CSC phenotypes, and matrix remodeling as the primary culprits.\r\n\r\nFinally, in aim 3, we utilized this tumoroid system to generate tumoroids with 23 different cell compositions to evaluate the role of TME cell composition in response to therapy. Drug assays with novel and traditional chemotherapies revealed that tumoroids with different compositions respond differently to therapy and that the number of monocytes included in the culture was associated with the greatest resistance to therapy. Furthermore, our random forest models trained on the drug responses of each cell composition were able to predict drug response with moderate success. With these models we identified that nuanced differences in cell composition can influence drug response and that the strongest predictor of response to therapy was the total quantity of non-cancer cells. Overall, this model demonstrates the potential of using the TME composition to predict patient drug response and direct clinical management.\r\n\r\nIn these aims we demonstrate the clear utility of complex and realistic, yet practical in vitro model systems in the study of chemoresistance and CSC maintenance in ovarian cancer. Specifically, we identified the link between CSCs and the development of chemoresistance in long term 3D in vitro serial passage culture. Furthermore, we showed that the non-cancer cells in the TME can confer chemoresistance and promote EMT associated with altered CSC phenotypes and matrix remodeling. Lastly, we demonstrated the potential of TME composition in predicting drug response. Overall, the model systems presented in this study provide platforms that can be used to better understand the role of CSCs and the TME in chemoresistance and poor clinical outcomes. This could ultimately lead to the development of novel therapies, enhanced clinical management, and improved clinical outcomes.\r\n\r\nDate: Wednesday, July 21, 2021\r\nTime: 9:00 AM\r\nZoom: https:\/\/umich.zoom.us\/j\/96111622879\r\nPassword: 326862\r\nChair: Dr. Geeta Mehta", "occurrence_notes":null, "guid":"84438-21623984@events.umich.edu", "permalink":"http:\/\/events.umich.edu\/event\/84438", "building_id":"null", "building_name":"Off Campus Location", "campus_maps_id":"1", "room":"", "location_name":"Virtual", "has_livestream":1, "livestream_type":"zoom", "livetream_require_login":0, "livestream_id":"96111622879", "livestream_password":"326862", "livestream_link":"https:\/\/umich.zoom.us\/j\/96111622879", "cost":"", "tags":["Basic Science","biomedical","biomedical engineering","Biosciences","bme","engineering","Medicine","Michigan Engineering"], "website":"", "sponsors":[ { "group_name":"Biomedical Engineering", "group_id":"1143", "website":"https:\/\/www.facebook.com\/profile.php?id=100094127215673" } ], "image_url":"https:\/\/events.umich.edu\/media\/attachments\/2021\/07\/event_84438_original-1.png", "styled_images":{ "event_thumb":"https:\/\/events.umich.edu\/media\/cache\/event_thumb\/media\/attachments\/2021\/07\/event_84438_original-1.png", "event_large":"https:\/\/events.umich.edu\/media\/cache\/event_large\/media\/attachments\/2021\/07\/event_84438_original-1.png", "event_large_2x":"https:\/\/events.umich.edu\/media\/cache\/event_large_2x\/media\/attachments\/2021\/07\/event_84438_original-1.png", "event_large_lightbox":"https:\/\/events.umich.edu\/media\/cache\/event_large_lightbox\/media\/attachments\/2021\/07\/event_84438_original-1.png", "group_thumb":"https:\/\/events.umich.edu\/media\/cache\/resolve\/group_thumb\/media\/attachments\/2021\/07\/event_84438_original-1.png", "group_thumb_square":"https:\/\/events.umich.edu\/media\/cache\/resolve\/group_thumb_square\/media\/attachments\/2021\/07\/event_84438_original-1.png", "group_large":"https:\/\/events.umich.edu\/media\/cache\/resolve\/group_large\/media\/attachments\/2021\/07\/event_84438_original-1.png", "group_large_lightbox":"https:\/\/events.umich.edu\/media\/cache\/resolve\/group_large_lightbox\/media\/attachments\/2021\/07\/event_84438_original-1.png", "event_large_crop":"https:\/\/events.umich.edu\/media\/cache\/resolve\/event_large_crop\/media\/attachments\/2021\/07\/event_84438_original-1.png", "event_list":"https:\/\/events.umich.edu\/media\/cache\/resolve\/event_list\/media\/attachments\/2021\/07\/event_84438_original-1.png", "event_list_2x":"https:\/\/events.umich.edu\/media\/cache\/event_list_2x\/media\/attachments\/2021\/07\/event_84438_original-1.png", "event_grid":"https:\/\/events.umich.edu\/media\/cache\/event_grid\/media\/attachments\/2021\/07\/event_84438_original-1.png", "event_grid_2x":"https:\/\/events.umich.edu\/media\/cache\/resolve\/event_grid_2x\/media\/attachments\/2021\/07\/event_84438_original-1.png", "event_feature_large":"https:\/\/events.umich.edu\/media\/cache\/event_feature_large\/media\/attachments\/2021\/07\/event_84438_original-1.png", "event_feature_thumb":"https:\/\/events.umich.edu\/media\/cache\/resolve\/event_feature_thumb\/media\/attachments\/2021\/07\/event_84438_original-1.png" }, "occurrence_count":1, "first_occurrence":21623984 } , "84388-21623688": { "datetime_modified":"20210701T145834", "datetime_start":"20210721T100000", "datetime_end":"20210721T160000", "has_end_time":1, "date_start":"2021-07-21", "date_end":"2021-07-21", "time_start":"10:00:00", "time_end":"16:00:00", "time_zone":"America\/Detroit", "event_title":"A Garden of Earthy Delights: Ceramic Sculptures by Ann Arbor Potters Guild at Matthaei Botanical Gardens", "occurrence_title":"", "combined_title":"A Garden of Earthy Delights: Ceramic Sculptures by Ann Arbor Potters Guild at Matthaei Botanical Gardens", "event_subtitle":"", "event_type":"Exhibition", "event_type_id":"7", "description":"Inspired by the synergy between nature\u2019s beauty and complexity, and in partnership with the University of Michigan Matthaei Botanical Gardens, artists from the Ann Arbor Potters Guild created work centered around Michigan\u2019s natural landscapes and specifically for the Great Lakes Garden at Matthaei. Potters Guild artists created eight free-standing sculptures using the full range of traditional ceramic methods and tools, the potter\u2019s wheel, press molds, coil building, slab construction, and sculpting. Depictions range from naturalistic and representational, to abstract, as well as a whimsical nod or two to the timeless role of fantasy and myth in humankind\u2019s communion with nature. Exhibit is free but scheduled reservations are required to visit the botanical gardens. Use this link to book your visit: https:\/\/www.signupgenius.com\/go\/8050f4aadad22a2fd0-matthaei1", "occurrence_notes":"Reservations required to visit the exhibit and the botanical gardens. Use this link: https:\/\/www.signupgenius.com\/go\/8050f4aadad22a2fd0-matthaei1", "guid":"84388-21623688@events.umich.edu", "permalink":"http:\/\/events.umich.edu\/event\/84388", "building_id":"0", "building_name":"Matthaei Botanical Gardens", "campus_maps_id":"554", "room":"", "location_name":"Matthaei Botanical Gardens", "has_livestream":0, "cost":"", "tags":["Art","Outdoors"], "website":"", "sponsors":[ { "group_name":"Matthaei Botanical Gardens & Nichols Arboretum", "group_id":"1072", "website":"" } ], "image_url":"https:\/\/events.umich.edu\/media\/attachments\/2021\/07\/event_84388_original-1.jpeg", "styled_images":{ "event_thumb":"https:\/\/events.umich.edu\/media\/cache\/event_thumb\/media\/attachments\/2021\/07\/event_84388_original-1.jpeg", "event_large":"https:\/\/events.umich.edu\/media\/cache\/event_large\/media\/attachments\/2021\/07\/event_84388_original-1.jpeg", "event_large_2x":"https:\/\/events.umich.edu\/media\/cache\/event_large_2x\/media\/attachments\/2021\/07\/event_84388_original-1.jpeg", "event_large_lightbox":"https:\/\/events.umich.edu\/media\/cache\/event_large_lightbox\/media\/attachments\/2021\/07\/event_84388_original-1.jpeg", "group_thumb":"https:\/\/events.umich.edu\/media\/cache\/group_thumb\/media\/attachments\/2021\/07\/event_84388_original-1.jpeg", "group_thumb_square":"https:\/\/events.umich.edu\/media\/cache\/group_thumb_square\/media\/attachments\/2021\/07\/event_84388_original-1.jpeg", "group_large":"https:\/\/events.umich.edu\/media\/cache\/group_large\/media\/attachments\/2021\/07\/event_84388_original-1.jpeg", "group_large_lightbox":"https:\/\/events.umich.edu\/media\/cache\/group_large_lightbox\/media\/attachments\/2021\/07\/event_84388_original-1.jpeg", "event_large_crop":"https:\/\/events.umich.edu\/media\/cache\/event_large_crop\/media\/attachments\/2021\/07\/event_84388_original-1.jpeg", "event_list":"https:\/\/events.umich.edu\/media\/cache\/event_list\/media\/attachments\/2021\/07\/event_84388_original-1.jpeg", "event_list_2x":"https:\/\/events.umich.edu\/media\/cache\/event_list_2x\/media\/attachments\/2021\/07\/event_84388_original-1.jpeg", "event_grid":"https:\/\/events.umich.edu\/media\/cache\/event_grid\/media\/attachments\/2021\/07\/event_84388_original-1.jpeg", "event_grid_2x":"https:\/\/events.umich.edu\/media\/cache\/event_grid_2x\/media\/attachments\/2021\/07\/event_84388_original-1.jpeg", "event_feature_large":"https:\/\/events.umich.edu\/media\/cache\/event_feature_large\/media\/attachments\/2021\/07\/event_84388_original-1.jpeg", "event_feature_thumb":"https:\/\/events.umich.edu\/media\/cache\/event_feature_thumb\/media\/attachments\/2021\/07\/event_84388_original-1.jpeg" }, "occurrence_count":79, "first_occurrence":21623668 } , "84567-21624162": { "datetime_modified":"20210805T063034", "datetime_start":"20210721T100000", "datetime_end":"20210721T200000", "has_end_time":1, "date_start":"2021-07-21", "date_end":"2021-07-21", "time_start":"10:00:00", "time_end":"20:00:00", "time_zone":"America\/Detroit", "event_title":"Virtual Hiring Event with a CSL Recruiter", "occurrence_title":"", "combined_title":"Virtual Hiring Event with a CSL Recruiter", "event_subtitle":"", "event_type":"Careers \/ Jobs", "event_type_id":"2", "description":"We are looking to identify new graduates, alumni and professionals for our Management Trainee Program. The Management Trainee (MT) Program is approximately 6 \u2013 7 months, and prepares Management Trainees (MTs) for an advancement opportunity into an Assistant Manager Position. MTs are partnered with an experienced and knowledgeable Manager of the same focal point, known as a Sponsor, who will guide them and help them prepare for a next level promotion opportunity. \n\nLeadership training is completed through the Pinnacle Program at one of our two training sites (Las Vegas, NV and Pinellas Park, FL). The Pinnacle Program offers a blended learning approach for new CSL Plasma center leadership, and incorporates self-directed, interactive, and classroom style methods to provide leadership training that is engaging, effective, efficient, and consistent. \nWe are seeking candidates that have:\n\u2022\tBachelor's degree preferred. If no degree, must have equivalent combination of education and professional work experience, including a minimum of one year supervisory, leadership experience responsible for overseeing the activities of others.\n\u2022\tAbility to relocate upon completion of the MT Program, within a maximum of nine months (a company relocation program is included)\n\u2022\tOpen and flexible availability (must be available to work all hours of operation and travel as required)\n\u2022\tAbility for overnight travel 1 \u2013 2 weeks at a time (approximately five weeks for training, and two weeks for travel assignments post program completion while waiting to secure next level promotion)\n\u2022\tExcellent communication skills \n\u2022\tEffective collaboration skills and ability to instill trust\n\u2022\tStrong ability to drive results and direct work\n", "occurrence_notes":null, "guid":"84567-21624162@events.umich.edu", "permalink":"http:\/\/events.umich.edu\/event\/84567", "building_id":"", "building_name":"", "campus_maps_id":"", "room":"", "location_name":"", "has_livestream":0, "cost":"", "tags":[], "website":"", "sponsors":[ { "group_name":"University Career Center UCC", "group_id":"1140", "website":"http:\/\/careercenter.umich.edu" } ], "image_url":"", "styled_images":{ "event_thumb":"", "event_large":"", "event_large_2x":"", "event_large_lightbox":"", "group_thumb":"", "group_thumb_square":"", "group_large":"", "group_large_lightbox":"", "event_large_crop":"", "event_list":"", "event_list_2x":"", "event_grid":"", "event_grid_2x":"", "event_feature_large":"", "event_feature_thumb":"" }, "occurrence_count":1, "first_occurrence":21624162 } }