Presented By: Biomedical Engineering
“Microfabricated Medical Devices for Diagnostics and Advanced Biological and Cellular Manipulations”
BME 500 Seminar Series: Jeffrey D. Zahn, PhD
Jeffrey D. Zahn, PhD
Associate Professor
Department of Biomedical Engineering
Rutgers, The State of University of New Jersey
“Microfabricated Medical Devices for Diagnostics and Advanced Biological and Cellular Manipulations”
ABSTRACT: The focus of research conducted in the Zahn laboratory is the design and fabrication of microfabricated devices for clinical diagnosis, treatment of disease and supportive culture devices. Batch fabricated microfluidic platforms that can mimic or improve upon conventional sample preparation techniques performed in laboratories hold great potential to enable both research and healthcare advances. These miniaturized diagnostic devices have been termed micro total analysis systems (μTAS) or biochips and combine sensing mechanisms (physical, optical, electrical or chemical) with microfluidics.
Dr. Zahn’s research combines analytical and numerical modeling of microscale phenomena with device design, fabrication, and testing of BioMEMS components in an adaptive and iterative process for device optimization. Several projects currently underway in the Zahn laboratory which will be discussed including:
1) the development of a ‘smart’ electroporator with continuous cell impedance monitoring that automatically detects, electroporates, and monitors individual cells for changes in permeability and delivery, dynamically modulating the pulse to prevent damaging over‐exposure that kills cells and useless underexposure that does not permeabilze a cell.
and
2) the development of a multicompartment tissue culture platform to recapitulate in vitro neurocircuitry models using human neuronal cells derived from induced pluripotent stem (IPS) cells. This culture system was designed to allow modeling the neuronal circuitry of the mesolimbic reward system by segregating excitatory (GLUT), inhibitory (GABA) and dopaminergic (DA) induced neuron cell bodies while maintaining axonal communication and synapse formation from one chamber into another through communicative microchannels. Several novel features of this system are: an open well design to perform patch clamp electrophysiology within the device, the use of optogenetics for selective cell stimulation, and the integration of human derived induced neurons (iNs).
Associate Professor
Department of Biomedical Engineering
Rutgers, The State of University of New Jersey
“Microfabricated Medical Devices for Diagnostics and Advanced Biological and Cellular Manipulations”
ABSTRACT: The focus of research conducted in the Zahn laboratory is the design and fabrication of microfabricated devices for clinical diagnosis, treatment of disease and supportive culture devices. Batch fabricated microfluidic platforms that can mimic or improve upon conventional sample preparation techniques performed in laboratories hold great potential to enable both research and healthcare advances. These miniaturized diagnostic devices have been termed micro total analysis systems (μTAS) or biochips and combine sensing mechanisms (physical, optical, electrical or chemical) with microfluidics.
Dr. Zahn’s research combines analytical and numerical modeling of microscale phenomena with device design, fabrication, and testing of BioMEMS components in an adaptive and iterative process for device optimization. Several projects currently underway in the Zahn laboratory which will be discussed including:
1) the development of a ‘smart’ electroporator with continuous cell impedance monitoring that automatically detects, electroporates, and monitors individual cells for changes in permeability and delivery, dynamically modulating the pulse to prevent damaging over‐exposure that kills cells and useless underexposure that does not permeabilze a cell.
and
2) the development of a multicompartment tissue culture platform to recapitulate in vitro neurocircuitry models using human neuronal cells derived from induced pluripotent stem (IPS) cells. This culture system was designed to allow modeling the neuronal circuitry of the mesolimbic reward system by segregating excitatory (GLUT), inhibitory (GABA) and dopaminergic (DA) induced neuron cell bodies while maintaining axonal communication and synapse formation from one chamber into another through communicative microchannels. Several novel features of this system are: an open well design to perform patch clamp electrophysiology within the device, the use of optogenetics for selective cell stimulation, and the integration of human derived induced neurons (iNs).
Explore Similar Events
-
Loading Similar Events...
