Scientific collections serve the global research community as storehouses of biodiversity information, and recent advances and increased access to Computed Tomography (CT) are opening up new avenues for integrative collections-based research. Through CT, we are able to generate three-dimensional volumes of external and internal anatomy for morphological descriptions, as well as quantitative analyses of surfaces and volumes such as with geometric morphometrics. As the method is not destructive, we can easily integrate rare and important specimens into our studies and can capture and quantify traits that otherwise would be difficult in traditional preparations, such as osteoderm distributions or cranial endocasts. Through contrast-enhanced microCT scanning we can also visualize soft tissues, such as muscles, nerves, and arteries, facilitating the study of complex anatomical systems. The images and 3D volumes that result form this work can be easily shared and repurposed for other forms of future research and education (including 3D printing). Over the next four years, 16 US institutions will collaborate to create the open vertebrate (oVert) network: an unprecedented library of vertebrate morphology that contains high-resolution 3D anatomy of over 20,000 museum specimens. These emerging digital 3D resources will renew interest in phenotypic diversity and evolution and make our scientific collections accessible to more people than ever before.