The Tepui region of South America contains some of the most remote and pristine forests on the planet. Mycological expeditions over the last 15+ years have yielded a cornucopia of unusual fungi including hundreds of new species and genera of macrofungi. This talk will cover the basics of conducting fieldwork in remote areas, and the contributions that only long term studies combined with specimen vouchering and various molecular approaches can bring to understanding the ecology and diversity of any system.