Transient activity of cholinergic projections from the basal forebrain to the prefrontal cortex is necessary for attentional performance and cue processing. Two lines of investigation aim to elucidate the neural mechanisms underlying cue-processing and cue-guided movement. The first of these examined choline transporter (CHT) activity in the prefrontal cortex of mice expressing the human CHT coding variant, I89V. As choline reuptake is the rate-limiting factor in acetylcholine synthesis, CHT impairment was hypothesized to have a strong influence on cholinergic function in attentional circuits. Separately, a second series of experiments evaluated glutamatergic cortico-striatal projections during cue-guided movement in rats. In this way, we hope to further illuminate the contribution of cholinergically-driven striatal inputs in the completion of the attentional-motor circuit.