Presented By: LSA Biophysics
Biophysics Virtual Seminar Series
Ph.D. Candidates Nirupama Sumangala & Keanu Guardiola Flores
Please see below for link to join the Zoom event. Passcode: Biophysics
The Biophysics Virtual Seminar Series Presents:
Nirupama Sumangala - Biophysics Ph.D. Candidate (Ramamoorthy Group)
"Lipid Membrane Plays an Important Role for Facilitating Electron Transfer in Cytochrome P450"
Abstract: Cytochrome P450s (CYP450s) are a ubiquitous superfamily of enzymes that play a vital role in the metabolism of many exogenous and endogenous substrates including over 70% of the drugs on the market. For the catalytic reaction, CYP450 requires two electrons to be subsequently delivered, with the first one coming from cytochrome P450 reductase (CPR) and the second one from either CPR or cytochrome b5 (cytb5). We are interested in understanding the role of membrane in mediating the electron transfer from redox partners to CYP450. Our findings reveal that the lipid membrane is crucial to mediate a productive CYP450-CPR complex for electron transfer. Our data emphasizes the importance of studying the structure, dynamics and kinetics of CYP450 metabolon in a biologically relevant membrane mimetic system.
Keanu Guardiola Flores - Biophysics Ph.D. Candidate (Wood Group)
"Drug Effects on Enterococcus Faecalis Biofilms: Growth, Topology, and Population Dynamics"
Abstract: The emergence of antibiotic resistance poses a growing threat to public health and increasingly limits our ability to treat and control infections. Recently, researchers have shifted their focus to length scales where ecological and evolutionary dynamics of bacterial communities highlight new approaches for slowing resistance with currently available drugs. By combining confocal microscopy with simple mathematical models I will show how antibiotics shape the composition of biofilms as well as their spatial architecture at the single-cell level. Our results suggest that in spatially structured populations, which may more accurately reflect natural bacterial communities, the selection of resistance is not a simple result of homogenous selections but depends critically on the spatial arrangement of cells.
The Biophysics Virtual Seminar Series Presents:
Nirupama Sumangala - Biophysics Ph.D. Candidate (Ramamoorthy Group)
"Lipid Membrane Plays an Important Role for Facilitating Electron Transfer in Cytochrome P450"
Abstract: Cytochrome P450s (CYP450s) are a ubiquitous superfamily of enzymes that play a vital role in the metabolism of many exogenous and endogenous substrates including over 70% of the drugs on the market. For the catalytic reaction, CYP450 requires two electrons to be subsequently delivered, with the first one coming from cytochrome P450 reductase (CPR) and the second one from either CPR or cytochrome b5 (cytb5). We are interested in understanding the role of membrane in mediating the electron transfer from redox partners to CYP450. Our findings reveal that the lipid membrane is crucial to mediate a productive CYP450-CPR complex for electron transfer. Our data emphasizes the importance of studying the structure, dynamics and kinetics of CYP450 metabolon in a biologically relevant membrane mimetic system.
Keanu Guardiola Flores - Biophysics Ph.D. Candidate (Wood Group)
"Drug Effects on Enterococcus Faecalis Biofilms: Growth, Topology, and Population Dynamics"
Abstract: The emergence of antibiotic resistance poses a growing threat to public health and increasingly limits our ability to treat and control infections. Recently, researchers have shifted their focus to length scales where ecological and evolutionary dynamics of bacterial communities highlight new approaches for slowing resistance with currently available drugs. By combining confocal microscopy with simple mathematical models I will show how antibiotics shape the composition of biofilms as well as their spatial architecture at the single-cell level. Our results suggest that in spatially structured populations, which may more accurately reflect natural bacterial communities, the selection of resistance is not a simple result of homogenous selections but depends critically on the spatial arrangement of cells.
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