Abstract: Diffusion tensor imaging (DTI) is a structural imaging technique that measures the orientation and direction of white matter fiber tracts in the brain. DTI is a useful tool to study white matter microstructure as it pertains to topics such as brain maturation, behavioral outcomes, and cognitive functioning. However, DTI has unique methodological challenges and a complex workflow. This presentation will provide a big picture overview of DTI, its research and clinical applications, and a walk-through of best practices for experimental analysis. Discussion topics will include quality control issues and troubleshooting, as well as the pros and cons of various DTI processing tools.