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Presented By: LSA Biophysics

Biophysics Seminar Series

Dr. Katrin Heinze (Universität Würzburg)

Dr. Katrin Heinze Dr. Katrin Heinze
Dr. Katrin Heinze
The Biophysics Virtual Seminar Series presents:

Dr. Katrin Heinze - Rudolf Virchow Center for Integrative and
Translational Bioimaging, Universität Würzburg

“Boosting high-resolution fluorescence by tunable nano-coatings"

ABSTRACT: The “Resolution Revolution" in fluorescence microscopy over the last decades has given rise to a variety of techniques that allow imaging beyond the diffraction limit with resolution up to the nanometer range. One particularly powerful technique is direct stochastic optical
reconstruction microscopy (dSTORM), a widely-used type of single molecule localization microscopy (SMLM), which is based on the temporal separation of the emission of individual fluorophores
and subsequent localization analysis. This eventually allows to reconstruct a super-resolved image revealing details down to typically 20 nm in a cellular setting. The key point here is the achievable localization precision, which mainly depends on the image contrast generated by the individual fluorophore’s emission. We found that reflective metal-dielectric nano-coatings represent a tunable nanomirror that can do both quenching and boosting fluorescence for high-contrast imaging on the nanoscale. Such mirror-enhanced fluorescence is very different from other surface effects based on total internal reflection microscopy or optoplasmonics. While surface-plasmon supported fluorescence methods provide much higher enhancement factors, mirror-enhanced approaches are more versatile and thus highly suitable for modern bio-imaging. The resolution improvement achieved with such mirror-enhanced STORM (meSTORM) is both spectrally and spatially tunable and thus allows for dual-color approaches on the one hand, and selectively highlighting region above the cover glass on the other hand. Even if the resulting resolution boost is based on a near-field effect and thus restricted to imaging near surfaces, a large variety of membrane fluorescence approaches even beyond SMLM benefit. Thus, live-cell
Fluorescence Correlation Spectroscopy and Fluorescence Resonance Energy Transfer are as well perfect candidates to be pushed to the next level.
Dr. Katrin Heinze Dr. Katrin Heinze
Dr. Katrin Heinze

Livestream Information

 Zoom
January 22, 2021 (Friday) 12:00pm
Meeting ID: 94897559686

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