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Presented By: Department of Chemistry

Activity-based chemical probes for RNA modifying enzymes

Ralph Kleiner (Princeton University)

Epitranscriptomic RNA modifications can regulate RNA activity, however there remains a major gap in our understanding of the scope of RNA chemistry present in biological systems, the enzymes responsible for regulating modification levels, and the ultimate function of these modifications in biological processes. To address this gap, our group has developed RNA-mediated activity-based protein profiling (RNABPP), a chemoproteomic strategy relying upon metabolic RNA labeling with modified nucleoside probes, RNA-protein enrichment, and quantitative proteomics. This lecture will discuss the application of RNABPP with fluoropyrimidine nucleosides in human cells in order to profile diverse families of RNA modifying enzymes including m5C and m5U methyltranferases, as well as dihydrouridine synthase (DUS) enzymes. Time allowing, I will also discuss new developments in RNA metabolic labeling technology.

Ralph Kleiner (Princeton University)

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