Molecular compartmentalization is vital for cellular physiology. For instance, proteins for intercellular adhesion and communication are localized to the cell surface while many energy-producing enzymes stay in the mitochondrion. High-resolution, proteome-wide mapping of protein localization is of core importance for understanding cellular organization and processes.

In this talk, Jiefu Li, Ph.D., will describe an end-to-end pipeline for in situ cell-surface proteomics, from chemical and transgenic tools to a user-friendly platform for data analysis, and present how in situ cell-surface proteomics of developing brains discovers “outside-the-box” regulators of neuronal wiring. Moreover, in vivo interactome mapping of key synaptic organizers uncovers the cellular and molecular mechanisms of synaptic matching and maturation and highlights the RhoGAP/RhoGEF as a signaling hub for synaptic development.

Speaker:
Jiefu Li, Ph.D.
Group Leader, 4D Cellular Physiology
Janelia Research Campus, Howard Hughes Medical Institute