Biomedical Engineering pres.
BME 500: Ruixuan Gao
“In Situ Nanoscale Imaging of Molecular Constituents and Functions in Cells and Intact Tissues”
In this talk, I will first introduce an optical imaging pipeline named expansion lattice light-sheet microscopy (ExLLSM) and its application to multiplexed, volumetric imaging of molecular constituents in cells and intact tissues. Using ExLLSM, our study has revealed molecular-specific structures of organelles, synapses, myelin sheaths, and neurites in rodent and insect brains at ∼60 by 60 by 90 nm effective resolution across dimensions that span millimeters. Next, I will present two recently developed methods that further extend the resolution and throughput of ExLLSM: (1) a non-radical hydrogel chemistry that forms a homogenous polymer network and physically separates biomolecules or fluorescent labels up to 40-fold linearly, and (2) a multi-modal optical microscopy that enables rapid, high-resolution imaging of both expanded and live tissues. Lastly, I will discuss the significance of these imaging methods in the context of microanatomy and functional omics.
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