DCMB Seminar Series pres.
DCMB / CCMB Weekly Virtual Seminar featuring Gioele La Manno, Ph.D. (EPFL Life Sciences Early Independent Research Scholar (ELISIR)
Revealing the brain’s molecular anatomy with single-cell and tomography-based spatial transcriptomics
Continuing in this direction, I will show the approach that we recently proposed as a general way to spatially resolve different types of next-generation sequencing data. We designed an imaging-free framework to localize high throughput readouts within a tissue by combining compressive sampling and image reconstruction. Our first implementation of this framework transformed a low-input RNA sequencing protocol into an imaging-free spatial transcriptomics technique (STRP-seq).
Finally, I will showcase the technique with the profiling of the brain of the Australian bearded dragon Pogona vitticeps. With this analysis, we revealed the molecular anatomy of the telencephalon of this lizard and provided evidence for a marked regionalization of the reptilian pallium and subpallium.
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